rapI [2017-8-11 16:0:3]

response regulator aspartate phosphatase, antagonist of ImmR

locus: BSU05010

pI: 5.05

mw: 46.40 kDa

protein length: 391 aa

gene length: 1173 bp

function: control of transfer of the mobile genetic element ICEBs1

product: response regulator aspartate phosphatase

essential: no

synonyms: yddL

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.2|Response regulator aspartate phosphatase]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.2|Mobile genetic elements] → [category|SW 5.2.1|ICEBs1]

Gene

Coordinates: 547,306 → 548,481

The protein

Catalyzed reaction/ biological activity

• binds ImmR and inhibits its activity, this results in induction of the genes for the transfer of the mobile genetic element ICEBs1 [Pubmed|17511812]

Protein family

• RAP family (according to Swiss-Prot)

Effectors of protein activity

• binding of [protein|F3E14B772A5DA9073ED806A9ADDD9B64F0DFDD5B|PhrI] indicates that neighbour cells do already contain the genetic element ICEBs1, this results in inactivation of RapI [Pubmed|17511812]

Structure

• [PDB|4I1A] [Pubmed|23526881]

Expression and Regulation

Operons

• genes: [gene|A208B4564A50D7BE2630DE40E8FAAF3C16236EE4|rapI]-[gene|F3E14B772A5DA9073ED806A9ADDD9B64F0DFDD5B|phrI]
  description: [Pubmed|11466295]

  sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|11466295], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11466295], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, [Pubmed|26582911], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]

  regulation

  • induced at high cell density ([SW|ComA]) [Pubmed|26582911]

References

Reviews

• 24995588

Original publications

• 11466295,17511812,23526881,26582911